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一般原则

冻干(真空冷冻干燥)多肽通常以稳定的粉末形式提供,并在研究使用前立即用稀释剂——通常是抑菌水——进行复溶,因为多肽溶液的降解速度比粉末形式快。

一般原则

无菌操作非常重要:在清洁环境中操作,每一步都使用全新的无菌注射器和针头,避免将污染物带入多肽小瓶或稀释剂小瓶中。稀释剂通常应缓慢加入,沿瓶壁内侧流下,而不是直接滴在粉末上,以避免产生过多泡沫,从而影响多肽结构。

本文未涵盖的内容

确切的稀释剂用量和浓度取决于具体产品、研究方案以及现有设备——本文有意不给出具体的复溶配方。请参阅您所用具体货号的检测证书/产品文档,如对您自己的研究用途有疑问,请直接联系我们的团队。

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